粘附分子P选择素在狼疮性肾炎中的作用

Significance of P-selectin Expression in Lupus Nephritis

采用酶联免疫吸附法、免疫组化及原位杂交检测35例狼疮性肾炎(LN)患者血浆和肾组织中 P 选择素表达水平。结果表明 LN 患者血浆中 P 选择素水平显著高于正常人,其中肾病综合征组和肾功能不全组水平显著高于轻型组和慢性肾炎组;P 选择素在肾组织中广泛表达,且在Ⅳ型和Ⅴ型 LN 肾小球中表达水平明显高于Ⅱ型和Ⅲ型,而它们在肾小管、肾间质的表达水平与小管间质病变程度密切相关。提示血浆和肾组织中 P选择素表达水平可成为判断狼疮性肾炎严重程度及预后的的指标。

P-selectin expression in plasma and renal tissues of 35 cases of lupus nephritis (LN) were detected by ELISA,immunohistochemistry and in situ hybridization.The results showed that plasma P-selectin level in LN patients is higher than controls.The level in patients with nephrotic syndrome or chronic renal failure was significantly higher than that in patients with slight changes or nephritis.Furthermore,up-regulated P-selectin expression was found in renal tissues,and glomerular P-selectin expression was significantly up-regulated in type Ⅳ and Ⅴ than type Ⅱ and Ⅲ.There was strong correlation between the degree of tubulointerstitial lesions and the expression of P-selectin on tubular epithelium or within interstitium.The study suggests that up-regulation of P -selectin expression in plasma and renal tissues may predict the progression of LN.