组织培养技术及其在脱毒马铃薯种薯生产中的应用

The tissue culture technology and its application in the production of the Virus-free potato seeds

马铃薯退化是由于无性繁殖导致病毒连年积累而形成,而马铃薯幼苗的生长点的生长速度大于病毒繁殖速度,利用这个生长时间差切取茎尖进行组织培养可获得脱毒苗,由脱毒苗快速繁殖可获得脱毒种薯。制取马铃薯脱毒苗的关键技术是茎尖培养、病毒检测、脱毒苗快繁。

Potato degradation results from the accumulating of viruses caused by the asexual reproduction for successive years. The seedlings of the potatoes grow much quicker than the viruses can multiply during the same period. By using the time gap between them, the stem tip can be dissected and cultivated. In so doing, the virus-free potato seedling can be obtained. The quick multiplication of the seedling leads to virus-free potato seeds. The key technologies in obtaining the virus-free seedling are the stem tip culturing, virus checking, and the proliferating of the virus-free seedling.