富含半胱氨酸的酸性分泌糖蛋白在病理性瘢痕中的表达及其意义

The Expression and Significance of Secreted Protein,Acidic and Rich in Cysteine in Hypertrophic Scar and Keloid Tissue

目的观察富含半胱氨酸的酸性分泌糖蛋白(Secreted protein,acidic and rich in cysteine,SPARC)在瘢痕疙瘩和增生性瘢痕中的表达规律及其意义。方法瘢痕患者的瘢痕疙瘩和3～12个月增生性瘢痕组织各26例;另取手术剩余的正常成人皮肤组织5例;所有标本用40 g/L甲醛固定,行连续切片,厚4μm,应用链菌素生物素-过氧化物酶标记物免疫组织染色法染色。同时设正常对照(正常皮肤)、阳性对照(胃癌癌变组织)、阴性对照(磷酸盐缓冲液代替一抗)。鼠抗人SPARC单克隆一抗浓度为1:200。结果正常成人皮肤中SPARC表达稀少,局限在真皮-表皮交界的乳头真皮,部分靠近基底膜血管和皮肤附件。SPARC在增生性瘢痕的真皮瘢痕组织、皮肤附件中低表达,且早期增生性瘢痕中表达无明显强于晚期增生性瘢痕(P>0.05)。在真皮中SPARC呈弥散性散在分布(同增生性瘢痕),阳性信号表达较低。增生性瘢痕及瘢痕疙瘩的SPARC较正常皮肤表达无差异(P>0.05)。结论SPARC在3～12个月增生性瘢痕中呈低表达,在瘢痕疙瘩中低表达。

Objective To investigate the expression and significance of Secreted protein, acidic and rich in eysteine （SPARC） in keloid and hypertrophic scar （HS）. Methods Tissue samples were harvested from 26 patients with keloid and 26 with HS （3-12 months） and 5 from the skin of healthy adults. All specimens were fixed in 40 g/L formalin. Then the specimens were continuously sliced into 4 Ixm thick sections. These sections were stained by streptoavidin-biotion method of immunohisto-chemistry. The other groups were normal control （normal skin）, positive control （gastric cancerous tissue） and negative control （phosphate buffer） instead of The first antibody. The concentration of Rat anti-human SPARC monoclonal antibody were 1：200. Results There was scarce expression of SPARC in the skin tissue of healthy adults, and it was only present in the dermal papillae at the dermis epider-mis conjunctions and partly in the blood vessels and skin appendages adjacent to the basement membrane. There was low expression of SPARC in the dermal scar tissue and skin appendages in HS. Also, early expression of hypertrophic scar sputum. There was no significantly difference in the expression between late hypertrophic scar（P〉0.05）. SPARC showed scattered diffuse distribution as the same in the hypertrophic scar, and the lower expression of positive signals in the keloid. Compared with that in normal skin, there was no significant difference in the SPARC expression between HS and keloid （P〉0.05）. Conclusion There was lower SPARC expression in HS （3-12 months）, and lower expression in keloid.