不同实验皮肤对青藤碱透过行为的影响

Effect of Different Animal Skin on the Transdennal Speed Constant of Sinomenine

目的:研究青藤碱透过不同实验皮肤的行为。方法:以改进型Franz扩散池为透皮吸收研究实验装置,pH6.8磷酸缓冲液为接收液,采用HPLC法测定试液中青藤碱透过不同实验皮肤的含量。结果:裸鼠的透皮速率常数与人皮较为接近,且重复性较好,其中贮存时间为0、3、7d的裸鼠皮肤的释药速率方程分别为Q=40.34t^(1/2)+19.6、Q=38.01t^(1/2)+25.3、Q=41.55t^(1/2)+24.7。结论:青藤碱透皮吸收研究宜选择裸鼠皮肤,且裸鼠皮肤在-20℃冰箱内贮存7天,对青藤碱的透皮吸收行为不会有明显影响。

Objective: To study the effect of different animal skin on the transdermal speed constant of sinomenine. Methods; Improved franz-cell was used for the transdermal study, pH6.8 phosphate buffer was used as the receptor and HPLC method was used for the determination of sinomenine through different animal skin. Results; The transdermal speed constant of the skin of hareless mice was much close to which of person. The higuchi equation of sinomenine through skin of hareless mice stored under -20℃ for 0d, 3d and 7d were Q = 40.34t1/2+196, Q = 38.01t1/2+ 25.3, Q = 41.55t1/2+ 24.7respectively. Conclusion; The results indicate that skin of hareless mice is suitable for the transdermal study of sinomenine, and the effect of storing time (0 -7d) under-40℃ is not obvious.