FcRL3基因与安徽籍汉族类风湿关节炎的相关性研究

Fc receptor like 3 in Han nationality of Anhui patients with rheumatoid arthritis

目的探讨Fc受体样基因3（FcRL3）部分单核苷酸多态性（SNP）在安徽籍汉族类风湿关节炎（RA）发病中的作用。方法选取RA患者140例，健康对照组187名。用Mass—Array TM SNP检测仪对FcRL3基因上的SNP位点：FcRL3-1（rs0158440）、FcRL3—2（rs2225828）、FcRL3—3（rs7528684）、FcRL3—4（rs11284799）、FcRL3—5（rs945635）、FcRL3—6（rs3761959）、FcRL3—7（rs2210913）、FcRL3—8（rs2282284）、FcRL3—9（rs2282283）SNP进行检测。单倍型构建采用PHASEv2．1软件完成。结果在RA组中FcRL3—3（rs75286841—169C等位基因的频率为43．80％，显著高于对照组33．33％，差异有统计学意义（X^2=7．348，P=0．007，OR=1．558，CI：1．130～2．150）。单倍型FcRL3—1～9 CCCGGGGAA、CTCGCAAAC、CCTACAAAA、CCTGCGGAA、CTCGCGAAC差异有统计学意义，其中RA组中FcRL3—1～9CCCGGGGAA、CTCGCAAAC单倍型频率明形高于时照组，FcRL3—1～9CCTACAAAA、CCTGCGGAA、CTCGCGAAC单倍型频率明屁低于对照组。结论FcRL3基因可能与安徽籍汉族RA的易感性相关。

Objective To investigate the association between a few single nucleotide polymorphisms （SNPs） in Fc receptor like 3 （FeRI,3） and rheumatoid arthritis （RA） in Han, nationality of Anhui patients. Methods One hundred and forty RA patients along with one hundred and eighty-seven healthy controls were included in the study. SIN Ps of FcRL3-1 （ rsO 158440 ）, FcRL3-2 （ rs2225828 ）, FcRL3-3 （ rs7528684 ）, FcRL3- 4（rs11284799）, FcRL3-5（rs945635）, FcRL3-6 （rs3761959）, FcRL3-7 （rs2210913）, FcRL3-8 （rs2282284）and FcRL3-9 （rs2282283） in the FcRL3 gene were genotyped by MALDI-TOF technology. Haplotypes were estimated using PHASE v 2.1 software. Results The frequency of FeRL3-3-169C alleles in RA patients was significantly increased compared with healthy controls（X^2=7.348, P=0.007, OR=1.558, CI： 1.130N2.150）. The frequency of FcRL3-1-9 CCCGGGGAA and CTCGCAAAC haplotypes were significantly increased and the frequency of FcRL3-1-9CCTACAAAA, CCTGCGGAA and CTCGCGAAC haplotypes were significantly decreased compared with healthy controls. Conclusion FcRL3 may be associated with RA susceptibility in Anhui Han population.