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转基因产品检测方法研究进展 被引量：12

Advance in detection methods of genetically modified organisms and their derived products
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摘要 综述了国内外转基因生物(Genetically modified organisms,GMOs)及其产品检测方法的研究进展,并重点介绍了目前广泛应用的一些检测方法。 The detection methods of genetically modified organisms and their products at home and abroad were described, and some methods widely used at present were emphatically introduced and evaluated.
作者 王荣谈 张建中 刘冬儿 张大兵 杨立桃
机构地区 上海瑞丰农业科技有限公司 上海市农业技术推广服务中心 上海交通大学生命科学技术学院
出处 《上海农业学报》 CSCD 北大核心 2010年第1期116-119,共4页 Acta Agriculturae Shanghai
关键词 转基因生物 核酸 蛋白质 检测方法 Genetically modified organisms Nucleic acid Protein Detection method
分类号 Q81 [生物学—生物工程]
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参考文献18

  • 1James C. Global Status of Commercialized Biotech[R]/GM Crops:2008 ISAAA Briefs,2008,No. 39. http: ffwww, isaaa, org/RESOURCES/publications/briefs/39/executivesummary/derault. html.
  • 2Brett GM,Chambers S J, Huang L, et al. Design and development of immunoassays for detention of proteins[J]. Food Control , 1999, 10(6):401 - 406.
  • 3Engvall E,Jonsson K, Perlmann P. Enzyme-linked immunosorbent assay. Ⅱ. Quantitative assay of protein antigen,immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes[J].Biochim Biophys Acta. 1971,28:251,427 - 434.
  • 4Holst-Jensen A,Ronning SB, Lovseth A, et al. PCR technology for screening and quantification of genetically modified organisms (GMOs)[J].Anal Bioanal Chem,2003,375,985- 993.
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  • 6Hardegger M,Brodmann P, Herrmann A. Quantitative detection of the 35S promoter and the NOS terminator using quantitative competitive PCR[J]. Eur Food Res Technol. 1999,209 : 83 - 87.
  • 7Vollenhofer S, Burg K,Schmidt J, et al. Genetically modified organisms in food-screening and specific detection by polymerase chain reaction[J]. J Agric Food Chem, 1999,47 : 5038 - 5043.
  • 8James D, Schmidt A, Wall E,et al. Reliable Detection and Identification of Genetically Modified Maize, Soybean, and Canola by Multiplex PCR Analysis[J]. J Agric Food Chem, 2003,51 : 5829 - 5834.
  • 9Germini A,Zanetti A,Salati C,et al. Development of a Seven-Target Multiplex PCR for the Simultaneous Detection of Transgenic Soybean and Maize in Feeds and Foods[J]. J Agric Food Chem, 2004,52 : 3275 - 3280.
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上海农业学报
2010年 第1期

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