骨髓细胞动员增强趋化因子活性对静脉血栓溶解吸收的影响

Effect of chemokines,activity enhanced by mobilization of bone marrow cells on solution and absorption of venous thrombosis

目的:探讨骨髓细胞动员对巨噬细胞聚集静脉血栓趋化作用的影响。方法:SD成年大鼠随机分为正常组、假手术组、血栓组和rhG-CSF组。术前0.5h、术后7d和14d采集外周血,用ELISA法检测外周血MCP-3水平。术后7d、14d取血栓组和rhG-CSF组下腔静脉标本,用RT-PCR法检测血栓中MCP-3mRNA的表达情况。结果:术后14drhG-CSF组外周血MCP-3水平明显高于术前,且高于假手术组和血栓组术后14d外周血MCP-3水平,分别与术前、假手术组和血栓组比较P<0.05,差异有统计学意义。术后7d和14d,rhG-CSF组MCP-3mRNA表达较血栓组显著升高,两组间比较有显著差异P<0.01。结论:骨髓细胞动员增强了MCP-3对巨噬细胞的趋化作用。

Objective：To discuss the effect of bone marrow mobilization on chemotaxis of aggregation of macrophages in venous thrombosis. Methods：SD adult rats were divided randomly into normal group, sham operation group, thrombns group and rhG-CSF group. Peripheral blood was harvested at 30rain before operation and on the 7th and ldth day Mter operation. Level of MCP-3 in peripheral blood was detected by ELISA. Inferior vena cava specimens of thromus group and rhG-CSF group were collected at postoperative 7th and 14th day. With the application of RT-PCR, expression of MCP-3mRNA in thrombus was detected. Results：The level of MCP-3 in peripheral blood of rhG-CSF group was significantly higher than that before operation and also higher than the levels of MCP-3 in peripheral blood of sham operation group and thrombus group on the 14th day after operation （P〈0.05）, and the difference was significant. On the 7th and 14th day after operation,compared with thrombus group, the expression of MCP-3 mRNA in rhG-CSF group remarkablely increased,and the difference was significant beween these two groups （P〈0.01）. Conclusion：Brone marrow cell mobilization enhances the chemotaxis of MCP-3 on macrophages.