肝细胞生长因子信号途径激活促进疟原虫子孢子感染

Promotion of plasmodium sporozoite infection by activation of hepatocyte growth factor signaling pathway

目的研究肝细胞生长因子及其信号通路激活与疟原虫肝细胞感染的关系。方法采用ELISA和Western blot来检测肝细胞生长因子。用FITC标记的葡聚糖吸收测定法检测肝细胞损伤,通过免疫荧光法观察肝细胞生长因子的表达与损伤的肝细胞的关系。同时,采用免疫共沉淀的方法检测肝细胞生长因子及其受体信号通路的激活以及磷酸化。结果疟原虫子孢子感染肝细胞后造成了肝细胞损伤,同时诱导了肝细胞生长因子的合成、表达和释放,肝细胞生长因子促使疟原虫子孢子感染肝细胞,肝细胞生长因子及其受体信号通路的激活是疟原虫子孢子感染肝细胞的必要前提。结论肝细胞生长因子及其受体可能是疟疾治疗的潜在靶点。

Objedive To investigate the relationship between plasmodium sporezoite infection of hepatocytes and the activation of hepatoeyte growth factor （HGF）signaling pathway. Methods The hepatocytes were infected by plasmodium sporozoites and HGF was detected by ELISA and Western blotting assay. Hepatoeyte injury was detected by FITC-dextran absorption assay and HGF was expressed in the same injury cells by immunofluorescence against HGF. In addition,activation of HGF ant its receptor signaling pathway were detected with immunopreeipitation and detection of phosphorylation status. Results The hepatocytes were inured by sporezoite migration and HGF was produced and HGF that rendered hepatocytes susceptible to plasmodium spomzoite infection. In addition,hepatocyte infections depended on activation of the hepatoeyte growth factor and its receptor signaling pathway. Conclusions The hepatocyte growth factor and its receptor may possibly be the potential targets for malaria treatment.