霉菌快速检测纸片法的研究

Study on a Rapid Paper Method of Mold Examination

食品中霉菌检测的国家标准方法为培养基平板法。我们研究了霉菌快速检测的纸片法。该技术为接种样品置（３６±１）℃４０～４８ｈ培养后，计数在纸片上生长的霉菌菌落数（同时生长醇母菌落），然后通过公式换算出每克（毫升）样品霉菌的菌落总数。采用该方法和国标法同时检测阳性菌种以及加主食品样品霉菌总数检测试验的比较，结果符合率高，统计学无显著性差异。认为霉菌快速检测纸片法具有快速、准确、方便等优点。

In southern China,molds is one of the key criteria for evaluating food hygiene,a new rapid paper method for mold examination was studdied.Filter patpers containing media were put in plastic boxes after drying.Liquid samples were ncubated on the paper and cultured at 36±1℃ for 40～48hrs.Then counted the colony of molds(and yeast )and calculated the real count per gram(mL)by a formula.By comparing the results of positive species and 189 food samples between the paper method and the traditional standard method,the results showed no statistical significant difference with high coincident rate.In conclusion,the paper method would be a quick,exact,scientific and convenient method.