小菊花色芽变品系的SRAP鉴定

SRAP Identification of Pink Flower Sport from Chrysanthemum with Small Flowers

利用SRAP技术进行小菊粉色花芽变与黄色花对照的DNA多态性分析。88对引物共扩增出清晰条带1169条,分子量在147～700bp之间。芽变品种及对照遗传相似系数为0.997,遗传物质多态性为0.68%,表明芽变与对照在遗传背景上高度的一致。筛选出5对引物,获得8条可能与花色芽变基因有关的特异条带,粉色花特有条带为190、550bp(Me4-Em10)、560bp(Me5-Em1)、410bp(Me6-Em7),对照特有条带为570、557bp(Me4-Em11)及220、350bp(Me6-Em5)。这些特异条带能够在植物生长早期鉴别芽变品种与对照。推测造成小菊粉色花芽变的原因很可能是染色体结构的变异。

Polymorphic DNA was studied in pink flower sport from chrysanthemum with small flowers using SRAP.A total of 1 169 clear bands wereamplified with 88 pair primer,the fragment length ranged from 147 bp to 700 bp.Genetic Similarity（GS）was 0.997,Genetic polymorphic was 0.68%.which showed high similarity in genetic between sport and control 5 pair primer amplified 8 distinctive bands,of which 190,550 bp（Me4-Em10）,560 bp（Me5-Em1）and 410 bp（Me6-Em7）was present only in sport,otherwise 570 bp,557 bp（Me4-Em11）and 220,350 bp（Me6-Em5）was only in control.These distinctive polymorphic DNA segments could be associated with flower color sport and identified pink flower lines from control one in the earlier stage.The flower-color-sport reason was maybe change in DNA structure.