达赛莱克特草莓离体培养快繁体系的建立

Study on Tissue Culture and Propagation System of DaSailaikete Strawberry

以达赛莱克特草莓品种的匍匐茎茎尖为起始材料,进行离体培养,分化出再生植株。通过试验,筛选出芽分化、继代增殖、生根的适宜培养基,系统的建立了草莓新品种较高频率的组培快繁体系。结果表明:以MS+6-BA0.5mg/L+NAA0.01mg/L培养基诱导产生不定芽,在MS+6-BA0.6mg/L+IBA0.06mg/L培养基上增殖系数较高,可达9.73倍,有效苗最多。用1/2MS+IBA0.5mg/L+NAA0.02mg/L+AC0.15%的生根培养基上,生根率高达100.00%。最佳练苗基质是腐殖土∶河沙为3∶1,移栽大田成活率达98%。

Adopted Dasailaikete Strawberry＇s stolon stem apex as the incept materials,in vitro cultivate and differentiate regeneration plants.Through further testing,filtrated appropriate medium for bud differentiation,proliferation and rooting medium,system established higher frequency of a new strawberry variety on tissue culture.The results showed ：the best medium for explants survival rate was 1/2MS.Used MS＋6-BA 0.5 mg/L＋NAA 0.01 mg/L medium to induce adventitious buds,In MS＋6-BA 0.6 mg/L＋IBA 0.06 mg/L medium propagation coefficient higher up to 9.73 times and had the most effective plants.Rooting with 1/2MS＋IBA 0.5 mg/L＋NAA 0.02 mg/L＋AC 0.15% medium,the rate was as high as 100.00%.Best practice was humus soil matrix Miller∶sand 3∶1,the survival rate got more than 90%,transplant field after two weeks,the survival rate was 98% and growing better.