四种补血草组织培养的比较研究

Comparative Analysis of Tissue Culture Efficiency in Four Varieties in Limonium

以叶片和带腋芽的花茎为外植体对不同补血草品种(P104、UH4、429、867)进行组织培养比较研究。结果表明:在培养基MS+6-BA0.3mg/L+NAA0.6mg/L上,品种均能通过带腋芽的花茎分化出芽,出芽率依次为P104>UH4>867>429,但污染严重;"情人草"品种P104、UH4能通过叶片诱导出愈伤组织,而"勿忘我"品种不能;在生根培养基1/2MS+IBA1.0mg/L+NAA0.2mg/L+Vc200mg/L+PP3330.2mg/L中,4个品种均能生根,其生根率依次为P104>UH4>429>867。

Blades and flower stalks with axillary buds were used as explants to compare the difference of tissue culture of four Limonium varieties（P104,UH4,429,867）by scales in vitro.The results showed that buds can be induced from the flower stalks with axillary buds of the four Limonium varieties can be obtained buds in the induction medium（MS＋6-BA 0.3 mg/L＋NAA 0.6 mg/L）.The induction rate order,from higher to lower level,was P104,UH4,867 and 429.But all of the varieties in the experiment had a high contamination rate.Blades of L.latlfolium varieties can be induced to from callus,but L.latlfolium varieties can not.At the same time,all the varieties can be form roots in the rooting mediun（1/2MS＋IBA 1.0 mg/L＋NAA0.2 mg/L＋Vc 200 mg/L＋PP333 0.2 mg/L）,but the root formation rates were significantly difference.