摘要
为研究重瓣长寿花的离体快繁技术,选用重瓣长寿花的花序作为外植体,以MS为基本培养基,比较添加4种浓度的6-BA和IAA对重瓣长寿花丛生芽诱导的效果。结果表明:花序在添加6-BA为2.5mg/L、IAA为1.0mg/L的适宜培养基下可直接诱导出大量丛生芽,经过继代培养,诱导生根,移栽可进行快繁。
With inflorescence as explants and MS as basic medium in tissue culture the effects of adding 4 concentrations of 6-BA and IAA on the induction of buds were compared to investigate the in vitro rapid propagation technology of Double-type Kalanchoe blossfeldiana.The results showed that the preferable medium of differentiation was MS supplemented with 2.5 mg/L 6-BA and 0.5 mg/L IAA.Then by secondary culture,rooting culture and transplanting the rapid propagation was established.
出处
《北方园艺》
CAS
北大核心
2010年第2期166-168,共3页
Northern Horticulture
关键词
重瓣长寿花
花序
丛生芽
组织培养
Double-type Kalanchoe blossfeldiana
inflorescence
bud
tissue culture
