合欢离体培养及高效再生体系的建立

Isolated Culture and Establishment of Regeneration System of Albizia julibrissin in Higher Efficiency

以合欢种子和当年生枝条为材料,研究了合欢离体培养及再生体系,探讨了不同激素组合对合欢愈伤组织的产生、分化及生根的影响,筛选出高效产生愈伤组织培养基为MS+BA2mg/L+KT0.5mg/L+NAA0.3mg/L;分化培养基为MS+BA1mg/L+NAA0.1mg/L;有利于生根的培养基为1/2MS+NAA0.5mg/L,以上培养基均加30g/L蔗糖、8g/L琼脂。

Studied the isolated culture and regeneration system of Albizia julibrissin,and discussed the effects of different hormone combination on callus,differentiation and rooting.The results showed MB＋BA 2 mg/L＋KT 0.5 mg/L＋NAA 0.3 mg/L＋30 g/L sucrose＋8 g/L agar was optimum medium for callus;MB＋BA 1 mg/L＋NAA 0.1 mg/L＋30 g/L sucrose＋8 g/L agar was optimum medium for differentiation;1/2MS＋NAA 0.5 mg/L＋30 g/L sucrose＋8 g/L agarwas the optimum medium for growing root.