单叶刺槐组织培养研究

Tissue Culture of Robinia. pseudoacaciaf. unifolia

以单叶刺槐(Robinia. pseudoacaciaf. unifolia)带腋芽的茎段为外植体,探讨不同植物激素6-BA、IBA、NAA对试管苗增殖以及植株再生的影响,建立单叶刺槐离体培养技术体系。结果表明:启动最适培养基为MS+6-BA1.0mg/L;增殖最适培养基为MS+6-BA1.0mg/L+NAA0.2mg/L;生根培养基为1/2MS+IBA50mg/L,预培养7d,再转至1/2MS0培养10d;在此体系中诱导率可达95.0%,增殖系数可达5.72,生根率83.3%,移栽成活率可达到100%。

Stems with axillaries bud of Robinia.pseudoacaciaf.unifolia were used as explants,this paper probed into the effects of 6-BA,NAA,IBA on proliferation and plantlet regeneration of Robinia.pseudoacaciaf.unifolia.The results showed that the medium suitable for bud germination was MS＋6-BA 1.0 mg/L;The optimum multiplication culture medium was MS＋BA 1.0 mg/L＋NAA 0.2 mg/L;The rooting culture medium was that the clones were cultured 7 d in 1/2MS＋IBA 50 mg/L,then were cultured 10 d in 1/2MS0 medium.In this system,the rate of induction of 95.0% the highest propagation coefficient was 5.72 and the rooting rate was 83.3% and the survived plantlets rate of transplant was 100%.