摘要
以5-’(AG)8(AGC)C-3’为引物对育种代号为82-6的平欧杂种榛品系的ISSR反应体系中各个主要影响因子进行优化筛选。结果表明:在20μL ISSR反应体系中各组分的最适浓度分别是,DNA模板为2.5 ng/μL,Mg2+浓度为1.875 mmol/L,1×buffer(不含MgCl2),引物的浓度为0.3μmol/L,Taq DNA聚合酶1 U,dNTP浓度为200μmol/L。并利用该优化体系对平欧杂种榛的15个品种(系)进行了ISSR扩增,证实该体系稳定可靠。
The optimization of main influential factors of ISSR reaction system about the ping＇ou breeding strain 82-6 was studied with primer 5＇-（AG）8 （AGC）C-3＇. The results showed that the optimum concentrations of components such as template DNA, Mg^2＋ , Buffer, primer, Taq DNA polymerase, dNTP in 20 μL reaction system were 2.5 ng/μL, 1.875 mmol/L, 1 ）〈 buffer （without Mg^2＋ ）, 0. 3 μmol/L, 1 U, 200 μmol/L. Amplifications of 15 ping＇ ou breeding varieties （strains）of hazelnut were achieved using this optimum system.
出处
《北方园艺》
CAS
北大核心
2010年第4期125-128,共4页
Northern Horticulture
基金
国家科技基础条件平台建设资助项目(2005DKA21002-07-29)
