利用通用旋转组合设计优化赤灵芝母种培养基的研究

The use of Universal Design Optimization Rotation of the Medium of Ganoderma lucidum Research Home

为解决赤灵芝生产中多次无性繁殖而常出现菌种老化和退化问题，优选适合赤灵芝母种的培养剂。利用四因素五水平二次通用旋转组合设计，研究葡萄糖、蛋白胨、硫酸镁、磷酸二氢钾对赤灵芝菌丝的长速、干重的影响。结果表明：葡萄糖、蛋白胨、酵母粉、无机盐（MgSO4·KH2PO4）4因素中，对赤灵芝菌丝长速的效应为葡萄糖〉蛋白胨〉酵母粉〉无机盐；对菌丝干重的效应为葡萄糖〉酵母粉〉无机盐〉蛋白胨。获得赤灵芝菌丝的长速〉1．31cm／d，菌丝干重〉26．09mg／tube，用葡萄糖16．37～17．05g／L，蛋白胨1．617～1．689g／L、酵母粉1．872～2．056g／L、无机盐（MgSO4·KH2PO4）2．352～2．706g／L。

To address the production of Ganoderrna lucidum on many occasions and often appear asexual strain aging and degradation, optimal for the cultivation of Ganoderma lucidurn Mother kinds of agents. The use of four-factor five-level design of the second general rotation studied glucose, peptone, yeast powder ,magnesium sulfate, potassium dihydrogen phosphate mycelium of Ganoderma lucidum and long speed, the impact of dry weight. Under the conditions of this experiment, glucose, peptone, yeast powder, inorganic salts （MgSO4 · KH2PO4 ） four factors, the speed of the Ganoderrna lucidum mycelia long the effects of glucose〉peptone〉yeast powder〉salts. The effect of the dry weight of glucose〉 yeast： powder〉salts〉peptone. Mycelium of Ganoderma lucidum was long speed〉1. 31 cm/d, dry weight of mycelium〉 26.09 mg/tube, and glucose 16.37-17.05 g/L,peptone 1. 617-1. 689 g/L, yeast powder 1.872-2. 056 g/L, inorganic Salt 2. 352-2. 706g/L.