化瘀温经利湿法治疗慢性盆腔炎20例临床观察

Therapy of Dissolving Blood Stasis,Warming Meridian and Removing Dampness in the Treatment of Chronic Pelvic Inflammatory Disease: A Clinical Observation of 20 Cases

目的观察化瘀温经利湿中药对慢性盆腔炎患者的疗效及对外周血CD4+CD25+CD127lo调节性T细胞(regulative T cells,Treg)的影响。方法将40例慢性盆腔炎患者按随机数字表法分为化瘀温经利湿中药治疗组20例和西药对照组20例,两组均以1个月为1个疗程,均治疗1个疗程,观察临床疗效及对症状出现率的影响;采用流式细胞术检测患者治疗前后外周血CD4+CD25+CD127loTreg的数量及其在CD4+T淋巴细胞中所占的比例;并检测治疗前后血沉(erythrocyte sedimentation rate,ESR)和C反应蛋白(C-re-active peptide,CRP)水平。结果与对照组比较,治疗组总有效率和CD4+CD25+CD127loTreg表达水平显著升高,ESR、CRP及症状出现率显著降低(P<0.05)。结论化瘀温经利湿中药治疗慢性盆腔炎的作用机制,可能与其上调CD4+CD25+CD127loTreg表达水平,促进免疫调节,降低炎症反应有关。

Objective To observe effects of traditional Chinese medicine of dissolving blood stasis, warming meridian and removing dampness in treating chronic pelvic inflammatory disease and its effects on peripheral blood CD4^＋ CD25^＋ CD127^lo regulatory T cells （Treg）. Methods According to random digits table, forty patients with chronic pelvic inflammatory disease were averagely randomized into treatment group （treated with traditional Chinese medicine of dissolving blood stasis, warming meridian and removing dampness） and control group （treated with western medicine）. The treatment course was one month. The clinical effects and symptoms frequency in both groups were observed after a treatment course. Before and after treatment, the number of peripheral blood CD4^＋ CD25^＋ CD127^loTreg and the ratio of CD4^＋ CD25^＋ CD127^lo Treg to CD4^＋ T lymphocytes were observed using flow cytometry, and the levels of erythrocyte sedimentation （ESR） and C-reactive peptide （CRP） were assayed. Results Compared with those in control group, the total efficiency and the expression level of CD4^＋ CD25^＋ CD127^lo Treg were significantly increased, the levels of ESR and CRP were significantly decreased （P〈0.05）. Conclusion The mechanism of traditional Chinese medicine of dissolving blood stasis, warming meridian and removing dampness in the treatment of chronic pelvic inflammatory disease may be related to increasing the expression level of CD4^＋ CD25^＋ CD127^lo Treg, promoting immunoregulation and decreasing inflammatory reaction.