绝经后妇女肾虚证与载脂蛋白E基因多态性的相关性

Relationship between Kidney Deficiency Syndrome and Apolipoprotein E Gene Polymorphism in Menopausal Women

目的探讨绝经后妇女肾虚证与载脂蛋白E(apolipoprotein E,ApoE)基因多态性的相关性。方法以80例绝经后肾虚证妇女及65例绝经后无肾虚证妇女为研究对象,以ApoE基因为候选基因,运用限制性片段长度多态性检测ApoE基因型。结果肾虚组人群ApoE基因型ε2/ε3、ε2/ε4、ε3/ε3、ε3/ε4和ε4/ε4频率分别为11.25%、8.75%、58.75%、11.25%、10.00%,对照组人群ApoE基因型ε2/ε3、ε2/ε4、ε3/ε3、ε3/ε4、ε4/ε4频率分别为15.38%、6.15%、69.23%、9.24%、0;肾虚组人群ApoE等位基因ε2、ε3、ε4频率分别为10.00%、70.00%、20.00%,对照组ε2、ε3、ε4频率分别为10.76%、81.55%、7.69%;肾虚组ApoE等位基因ε4频率明显高于正常对照组(χ2=4.386,P<0.05)。结论ApoE等位基因ε4频率升高与绝经后妇女肾虚证相关,可能是肾虚证的危险因子;ApoE基因型ε4/ε4是绝经后妇女肾虚证一个有用的标志物。

Objective To study the correlation between kidney deficiency syndrome and apolipoprotein E （ApoE） gene polymorphism in postmenopausal women. Methods Eighty postmenopausal women with kidney deficiency syndrome and sixty-five postmenopausal women without kidney deficiency syndrome were selected. The ApoE genotypes were detected using restriction fragment length polymorphism.Results Frequency of ApoE genotype ε2/ε3, ε2/ε4, ε3/ε3, ε3/ε4 and ε4/ε4 in kidney deficiency syndrome group was 11.25%, 8.75%, 58. 75%, 11.25%, 10.00%, respectively, which was 15.38%, 6%. 15%, 69.23%, 9.24% and 0 in control group, respectively. Frequency of ApoE allele ε2, εa and ε4 in kidney deficiency syndrome group was 10.00%, 70.00% and 20.00%, respectively, which was 10.76%, 81.55%, 7.69% in control group, respectively. Frequency of ApoE allele ε4 in kidney deficiency syndrome group was significantly higher than that in control group （χ^2 =4. 386, P〈0.05）. Conclusion The increased frequency of ApoE Allele ε4 is related to kidney deficiency syndrome in postmenopausal women, and it may be a risk factor for kidney deficiency syndrome. ApoE genotype ε4/ε4 is a useful marker of kidney deficiency syndrome in postmenopausal women.