胆囊结石形成时Calponin、VIP在胆囊平滑肌和Oddi括约肌中的表达及意义

Changes of vasoactive intestinal polypeptide and Calponin expression in gallbladder smooth muscle and Oddi sphincter in rabbits with cholesterol gallstone

目的探索血管活性肠肽(VIP)和调宁蛋白(Calponin)在胆囊平滑肌和Oddi括约肌中的表达,并评估二者在胆囊胆固醇结石形成机制中的作用。方法(1)动物模型:新西兰兔40只,随机分成对照组20只,喂普通饮食;结石组20只,饲喂含1.2g/d胆固醇的成石饮食4周。免疫组化法测定Calponin、VIP在胆囊平滑肌和Oddi括约肌中的表达。结果结石组16只出现胆囊结石,19只出现胆固醇结晶,对照组无结石及结晶出现;结石组胆囊平滑肌Calponin和VIP阳性产物与对照组相比较明显升高,差异有统计学意义(P<0.05),Oddi括约肌Calponin和VIP阳性产物与对照组相比较明显降低,差异有统计学意义(P<0.05)。Calponin阳性表达与VIP呈正相关。结论胆囊排空功能在胆囊结石形成中起重要作用,胆囊结石家兔胆囊排空障碍与Calponin、VIP明显相关。

Objective To study the role of vasoactive intestinal polypeptide（VIP） and Calponin in the pathogenesis of cholecystolithiasis. Methods Two dietary groups of New Zealand rabbits with 20 in each were allocated 1.2 g/d high cholesterol diet and common diet for 4 weeks. Results Comparing with the negative result in control group,cholesterol crystals and stones were found in 19 and 16 out of 20 gallbladders, respectively, under the polarizing microscope in lithogenic group. The positive expression of VIP and Calponin in gallbladder smooth muscle in lithogenic group was much higer than those in control group（P〈0.05）. The positive expression of VIP and Calponin in Oddi sphincter in lithogenic group was much lower than those in control group（P〈0.05）. The positive expression of VIP had a significant relation with Calponin（P〈0.05）. Conclusion The disorder of Calponin and VIP regulation, which induces gallbladder function disorder, plays an important role in gallstone formation.