电针对脑缺血再灌注大鼠颈静脉血糖和乳酸的影响

Effects of electroacupuncture on levels of the jugular venous blood glucose and blood lactate of rats with cerebral ischemia-reperfusion injury

目的观察脑缺血再灌注损伤后大鼠颈静脉血糖和乳酸的变化，以及电针对大鼠颈静脉血糖和乳酸的影响。方法雄性SD大鼠24只，随机分为假手术组（Sham）、脑缺血再灌注组（CI／R）及脑缺血再灌注＋电针组（CI／R＋EA），每组8只。采用4-VO法建立大鼠全脑缺血模型，于再灌流开始后，CI／R＋EA组电针“百会”“命门”和“足三里”穴，电针参数：频率30～50Hz，间断疏密波，电流强度1mA，以局部肌肉轻微震颤为度，时间20min。在缺血后24h，从右颈静脉抽取05mL血样，进行血糖和乳酸测定。结果CI／R组大鼠脑静脉血糖水平明显高于假手术组（P〈0．05），乳酸值显著降低（P〈0．05）；电针治疗后，CI／R＋EA组脑静脉血糖明显降低，乳酸水平升高，与CI／R组比较差异有统计学意义（P〈0．05）。结论针刺治疗可明显改善脑缺血再灌注大鼠的糖代谢，增加脑细胞对葡萄糖的摄取和利用。

Objective To observe the change of the jugular venous blood glucose and blood lactate of rats with cerebral ischemia - reperfusion injury and the effects of electroacupuncture on levels of the jugular venous blood glucose and blood lactate of the rats. Methods Twenty four male SD rats were divided randomly into sham operation （sham）, CI/R and CI/R ＋ EA groups with eight rats in each group. Transient general brain ischemia was induced by four - vessel occlusion and reperfusion. Elk （30 to 50 Hz, 1 mA） was applied to Baihui（GV20）, Mingmen（GV4） and Zusanli（ST36） for 20 rain after CI/R. Right jugular vein was cannulated to withdraw 0.5 mL blood samples for analysis blood gas and lactate during the postischemia 24 h. Results The level of the jugular venous blood glucose after ischemia- reperfusion in CI/R group increased but lactate decreased significantly than that in sham group （P 〈 0.05）. While compared with CI/R group, the levels of the jugular venous blood glucose decreased but lactate increased remarkably in CI/R ＋ EA group （ P 〈 0.05）. Conclusion The treatment with electropuncture can improve the glycometabolism of rats with cerebral ischemia- reperfusion injury and facilitate the brain cell to absorb and utilize glucose.