氟伐他汀对缺氧复氧处理的猪颈总动脉内皮细胞氧化还原功能及ICAM-1表达的影响

Effect of fluvastatin on oxidation-reduction function and expression of ICAM-1 in cultured pig carotid artery endothelial cells with hypoxia/reoxygenation injury

目的探讨3-羟-3-甲基戊二酸单酰辅酶A(HMG-CoA)还原酶抑制剂在缺血再灌注过程中的内皮保护作用。方法培养猪颈动脉血管内皮细胞(PCAEC),以0.1μmol/L、0.2μmol/L、0.5μmol/L、1.0μmol/L的氟伐他汀(来适可)共生长44 h,经缺氧1 h复氧3 h处理,以血清免疫学及细胞免疫化学方法测定MTT、GSH-PX、MDA、SOD、ICAM-1的值,观察氟伐他汀对缺氧复氧(H/RI)处理的猪颈总动脉内皮细胞氧化还原功能及细胞间黏附因子-1(ICAM-1)表达的影响。结果0.5μmol/L氟伐他汀干预的H/RI后细胞活力与单纯H/RI组有明显差别(P=0.01);H/RI可明显降低培养细胞的SOD活性,产生MDA较氟伐他汀组及正常对照组间有明显升高(P=0.001),正常对照组及氟伐他汀组与H/RI组间GSH-PX对照有明显差别(P=0.002);ICAM-1细胞免疫化学染色各组间均有显著差别(P=0.018)。结论适当浓度的氟伐他汀对缺氧复氧的内皮细胞功能具有保护作用。

Objective To observe the effect of fluvastatin on oxidation-reduction function and the expression of ICAM-1 in cultured PCAEC with H/RI and to study the pleiotropic effects of 3-hydroxy-3-methylglutaryl coenzyme A（HMG-CoA）reductase inhibitors beyond cholesterol reduction.Methods and Results PCAEC was separated by collagenase I and cultured in five groups.Select four groups at random.Treat each group with 0.1 μmol/L、0.2 μmol/L、0.5 μmol/L、1.0 μmol/L of fluvastatin before being H/R injuried.Examine MTT、GSH-X、MDA、SOD、ICAM-1（mRNA）after 1 h hypoxia and 3 h reoxygention.Conclusions Fluvastatin can protect the function of endothelial cells with hypoxia/reoxygenation injury.