昆明山海棠对中国仓鼠V79细胞核/质分裂协调性的影响

Disturbance of Mitotic Coordination by Tripterygium Hypoglaucum(Level) Hutch in V79 Chinese Hamster Cells

本研究以哺乳动物非整倍体诱发剂昆明山海棠根部水抽提物（ＴｒｉｐｔｅｒｙｇｉｕｍＨｙｐｏｇｌａｕ－ｃｕｍ（Ｌｅｖｅｌ）Ｈｕｔｃｈ，ＴＨＨ）处理中国仓鼠Ｖ９７细胞，通过检测Ｖ７９细胞末期和早Ｇ１期核／质构型变化和双核间期细胞的频率，分析了ＴＨＨ对哺乳动物细胞质分裂的影响。结果指出：ＴＨＨ能显著诱发Ｖ７９细胞的异常质裂（Ｐ＜０．００１），并明显的提高双核细胞的频率（Ｐ＜０．００１－０．０５）。结合以往的工作，提示ＴＨＨ不仅对哺乳动物染色体正常分离有直接的影响，同时对细胞质的正常分裂也具有显著抑制作用。受试物可能通过多途径诱发哺乳动物非整倍体。

V79 Chinese hamster cells were treated in vitro by Tripterygium Hypoglaucum (Level) Hutch(THH), Which contains some aneugen. The disturbance of mitotic coordination by THH were determined by examining nucleolus/cytoplasmic configuration of cells in late telophase-very early G 1 and the frequency of binuclear cells in interphase cells. The experiment showed that the compound influenced mitotic coordination and significantly increased the frequency of binuclear cells. The result indicated that THH contains some component which inhibits cytokinesis during mammal cell mitosis. According to the research before, we may conclude that THH not only directly induces chromosome non-disjunction but also shares another mechenisms of aneuploid induction related to cytokinesis in mammalian cells.