缺血预处理间充质干细胞移植治疗心肌梗死实验研究

Effect of the transplantation of hypoxic preconditioned mesenchymal stem cells on myocardial infarction

目的探讨静脉移植缺血预处理的间充质干细胞(MSCs)对梗死心肌的修复作用。方法选择纯种大白兔46只制作心肌梗死模型,分为缺氧预处理MSCs移植组(A组)、非缺氧预处理MSCs移植组(B组)和对照组,于心肌梗死后30 d分别检测心功能、梗死心肌的形态学变化以及新生毛细血管密度。结果与对照组比较,A、B组左心室收缩末期内径、舒张末期内径降低,心功能提高,室壁厚度增加,心肌梗死面积缩小,梗死区和梗死边缘区新生毛细血管密度增加(P<0.05)。结论MSCs移植治疗兔心肌梗死可改善心功能、缩小梗死面积、增加梗死心肌新生毛细血管密度,经缺氧预处理的MSCs移植治疗更有优势。

Objective To investigate the effect of the transplantation of hypoxic preconditioned mesenchymal stem cells （MSCs） on myocardial infarction. Methods Forty-six rabbits were randomly divided into three groups, including the transplantation of hypoxic preconditioned MSCs group （group A）, the transplantation of non-hypoxic preconditioned MSCs group （group B） and control group. Cardiac function, morphology and capillary density were detected on the 30th day of post-infarction. Results There was significant difference in cardiac function, infarct size, ventricle wall thickness and capillary density in group A and B compared with control group. Conclusions The transplantation of MSCs can promote cardiac function, reduce the infarct size and increase the capillary density in myocardial infarction rabbits, furthermore the hypoxic preconditioned MSCs is superior.