隐性脊柱裂脊髓内NOS1、脂肪及尼氏体含量的组织学分析

Analysis of NOS1,lipid and Nissl body in the spina bifida occulta spinal cord

目的检测隐性脊柱裂(SBO)胎鼠脊髓内一氧化氮合酶1(NOS1)、脂肪及神经元内尼氏体含量,探讨SBO发病机制。方法建立SBO胎鼠动物模型,通过免疫荧光染色方法测定脊髓内NOS1阳性细胞数量,油红O染色观察脊髓内脂肪含量,甲苯胺蓝染色观察脊髓神经元内尼氏体含量及神经元形态。结果SBO胎鼠脊髓中NOS1阳性神经元增多,神经元总数减少,细胸质内尼氏体减少或缺失,光密度值下降(P<0.01),脂肪含量无统计学差异(P>0.05)。结论SBO胎鼠腰骶段脊髓中NOS1分泌增多,尼氏体含量减少,可能是SBO出现临床症状的病理学基础之一。

Objective To detect the amount of neuronal nitric oxide synthase 1 （ NOS1 ）, lipid and Nissl body in the spinal cord of spina bifida occulta （SBO） fetal rats and to approach its pathogenesy. Methods Rats in experimental group was established SBO fetal rat model. The expression of NOS1 was determined by immunofluorescence staining and the amount of lipid in spinal cord was determined by oil red O staining, while the amount of Nissl bodies in neurons was determined by toluidine blue staining. Results In comparison to the normal group, the NOS1 positive motoneurons were significantly increased in the malformation spinal cord. No significant difference of the amount of fat tissue in the spinal cord was observed between experimental group and normal group. The toluidine number decreased in the malformation specimens （P 〈 0. O1 ）. Conclusion It may be one of the important pathological bases of SBO that the amount of NOS1 in spinal cord increases, whereas Nissl body decreases.