摘要
目的探讨加味补肝汤对实验性糖尿病大鼠周围神经病变的防治作用。方法以链脲佐菌素诱发糖尿病模型。分别于加味补肝汤治疗后4周、8周两个时间点处死大鼠,RT-PCR技术测定坐骨神经c-jun、蛋白激酶CβⅡ(PKC-βⅡ)mRNA的表达,免疫组织化学法测定脑源性神经生长因子(BDNF)蛋白的表达。结果加味补肝汤可降低c-jun、PKC-βⅡmRNA表达,上调BDNF蛋白表达(P<0.05)。结论加味补肝汤可减轻实验性糖尿病大鼠氧化应激反应,对糖尿病周围神经病变有较好的防治作用。
Objective To explore the effect of Jiaweibugan decoction on peripheral neuropathy in experimental diabetic rats. Methods Forty diabetic rats were induced by 1% streptozotocin in which 20 rats were cured by Jiaweibugan decoction. Rats in each group were killed on 4th or 8th week after the beginning of treatment. The c-jun and PKC-β II mRNA of sciatic nerve were detected by using RT-PCR and the brain-derived neurotrophic factors （BDNF） protein of sciatic nerve was detected by method of immunohistochemistry assay. Results The expression of c-jun and PKC-β II in rats treated by Jiaweibugan decoction were lower than those in non-treated model rats （ P 〈 0.05 ）. The expression of BDNF protein in rats treated by Jiaweibugan decoction were higher than those in non-treated model rats （P 〈 0.05）. Conclusion Jiaweibugan decoction can reduce the oxidative stress reaction and has a preventive and therapeutic effect on peripheral neuropathy in experimental diabetic rats.
出处
《山东医药》
CAS
北大核心
2010年第4期9-11,共3页
Shandong Medical Journal
基金
湖南省卫生厅中医药科研经费资助项目(6202-38)
湖南省重点学科建设经费资助项目[湘教通(2001)179号]
