消痰散结方对裸鼠人胃癌细胞增殖相关蛋白表达的影响

Influence of Xiaotansanjiefang on Expression of Human Gastric Cancer Proliferation Related Proteins

目的:探讨消痰散结方抑制胃癌细胞增殖的作用机制。方法:采用OB胶粘贴法建立裸鼠人胃癌MKN-45原位移植瘤模型,随机分成模型组,消痰散结低、中、高剂量组,5-Fu组,每组8只,观察消痰散结方干预胃癌组织NF-κBP65蛋白、COX-2蛋白表达的影响及相关性。结果:①消痰散结方中、高剂量组、5-Fu组NF-κBP65蛋白的阳性表达与模型组相比P<0.05,有统计学意义;②消痰散结方中剂量组COX-2蛋白阳性表达率与模型组相比P<0.05,有统计学意义,消痰散结方高剂量组COX-2蛋白阳性表达率与模型组相比有显著统计学差异,P<0.01;③胃癌细胞中NF-κBP65蛋白与COX-2蛋白表达存在正相关(r=0.952,P<0.01)。结论:胃癌MKN-45细胞中存在着活化的NF-κB及高表达的COX-2蛋白,并且存在线性相关,消痰散结方可能通过其抑制NF-κB活性,阻断了NF-κB对COX-2的活性调控,使胃癌细胞增殖受到抑制,从而阻断胃癌发展,控制胃癌复发与转移。

Objective： To discover the mechanism of Xiaotansanjiefang（XTF）,a traditional Chinese medicinal empirical Prescription for curing gastric cancer,in inhibiting proliferation of gastric cancer cells.Methods： Using OB glue paste method,the nude mice human gastric cancer MKN-45 in situ transplantation tumor model was established,based on which 40 mice were randomly divided into 5 groups（8 mice /every group）： the blank model group,low-dose XTF group,intermediate-dose XTF group,high-dose XTF group,and 5-Fu group.To observe its influence and correlation of Xiaotansanjiefang intervening gastric cancer NF-κBP65 protein and hTERT protein expression.Results：①There was a statistical meaning by comparing the intermediate-dose XTF group,high-dose XTF groups and 5-Fu group on NF-κBP65 protein positive expression rate with the model group,P〈0.05.②There was a statistical meaning by comparing the mid-dose Xiaotansanjie group with the model group on COX-2 protein positive expression rate（P〈0.05） The...更多re was a significant statistical meaning by comparing the high-dose Xiaotansanjie group on COX-2 protein positive expression rate with the model group,P〈0.01.③NF-κBP65 protein and hTERT protein expression in gastric Cancer cells is a positive correlation（r=0.952,P〈0.01）.Conclusion：There are activated NF-κBP65 protein and hTERT protein in the gastric cancer MKN-45 cell,and show linear correlation.Xiaotansanjiefang can betterly inhibit gastric cancer cells proliferation,thus blocking the development of gastric cancer,control of gastric cancer recurrence and metastasis.