摘要
目的探讨凋亡抑制基因survivin在胃癌组织中的表达。方法应用反转录-聚合酶链反应(RT-PCR),免疫组织化学染色技术研究Survivin mRNA在胃癌及正常胃组织中的表达。结果8例胃癌及3例正常胃黏膜中Ssurvivin的表达,3例正常胃黏膜组织中survivin基因无表达,而在8例胃癌组织中survivin基因阳性表达5例,阳性率为63%,差异具有统计学意义(P<0.05)。Survivin表达与胃癌组织的分化程度、淋巴结转移及肿瘤的TNM分期具有相关性,差异具有统计学意义(P<0.05)。结论survivin基因在胃癌组织中表达率升高,提示Survivin对胃癌发生、发展起重要作用,其过度表达可能提示胃癌预后不良。检测胃癌组织中survivin基因的表达可作为评价胃癌生物学行为和判断预后的重要指标。
Objective To investigate the expression of survivin gene （inhibitor of apoptosis） in gastric carcinoma tissues. Methods The expression of survivin mRNA in gastric carcinoma and normal gastric tissues was examined by RT- PCR and immunohistochemistry technology. Results The survivin protein could be detected in the gastric mucosa of 8 patients with gastric carcinoma and 3 normal cases, but the survivin gene could not be detected in the gastric mucosa of the 3 normal cases, and could be detected in 5 patients of the 8 patients with gastric carcinoma, the positive rate of survivin gene in patients with gastric carcinoma was 63%, and the the positive rate between normal cases and patients with gastric carcinoma was different （P〈0.05）. The expression of survivin gene was related to the degree of differentiation of gastric carcinoma, lymph node metastasis and TNM tumor staging （P〈0.05）. Conclusion The expression level of survivin gene could elevate in gastric carcinoma tissue, indicating that the su^ivin gene could play an important role in the development of gastric carcinoma. Snrvivin gene could be used as an indicator to evaluate the biological behavior and prognosis of gastric carcinoma.
出处
《实用医技杂志》
2010年第3期214-215,共2页
Journal of Practical Medical Techniques
