高效液相色谱法测定不同产地白花蛇舌草中齐墩果酸和熊果酸的含量

HPLC determination of oleanolic acid and ursolic acid in Hedyotis diffusa Willd from different sources

目的分析不同产地白花蛇舌草中齐墩果酸和熊果酸含量,为药材及其制剂的质量控制提供参考。方法采用高效液相色谱法(HPLC)测定。色谱柱AgilentC18柱,流动相为甲醇-水-冰醋酸-三乙胺(91.5∶8.5∶0.04∶0.1),检测波长210nm,流速0.80ml/min,柱温20℃。结果齐墩果酸和熊果酸分别在20.4～326.4μg/ml(r=0.9991)和39.2～627.2μg/ml(r=0.9994)范围内线性关系良好;齐墩果酸和熊果酸的平均回收率在98.0%～99.0%,相对标准差(RSD)分别为0.87%和1.22%。结论本方法准确、简便、重现性好,可同时测定白花蛇舌草中齐墩果酸和熊果酸的含量,能够应用于药材及其制剂的质量控制。

Objective To determine the oleanolic acid and ursolic acid in Hedyotis diffusa Willd from different sources and to provide a reference for quality control of raw materials and preparations. Methods The analysis was performed by HPLC with C18 column. The mobile phase consisted of methanol-water-glacial acetic aeid-triethylamine （91.5：8,5：0.04：0.1） with detection wavelength at 210 nm, the flow rate was 0.80 ml/min and the column temperature was maintained at 20 ℃. Results The linear range of oleanolic acid and ursolic acid were 20.4-326.4 μg/ml （r= 0.9991） and 39.2-627.2μg/ml （r=0.9994） respectively. The average recovery of oleanolic acid and ursolic acid were 98.0-99.0%, with RSD being 0.87% and 1.22% respectively. Conclusion The method developed in this study appeared accurate, convenient and reproducible and can be used for quality control of oleanolie acid and ursolic acid raw materials or preparations of Hedyotis diffusa Willd.