尿中MMP-9及MMP9-NGAL复合物对筛查乳腺疾病的临床意义

The clinical significance of urinary MMP-9 and MMP-9/NGAL complex in breast disease in female population

目的检测妇女尿中MMP-9及MMP-9/NGAL复合物,对乳腺疾病是否具有筛查作用。方法采用明胶酶底物电泳法,检测正常女性(60例)、乳腺良性疾病病人(41例)、乳腺癌病人(127例)尿中MMP-9及MMP-9/NGAL复合物的表达。结果乳腺疾病(乳腺良性疾病+乳腺癌)尿中MMP-9及MMP-9/NGAL复合物,与正常女性比较差异非常显著(P<0.01)。乳腺癌病人尿中MMP-9及MMP-9/NGAL复合物,与正常女性比较差异非常显著(P<0.01)。乳腺良性疾病与正常女性比较差异有显著性(P<0.05)。结论检测尿中MMP-9及MMP-9/NGAL复合物,对乳腺疾病的筛查具有重要意义。尤其对乳腺癌筛查更具意义。

Objectives To investigate the potential of urinary MMP-9 and MMP-9/NGAL complex as screening breast disease in female population. Methods Urinary MMP-9 and MMP-9/NGAL complex were detected with substrate zymography gel eleetrophoresis. Urine samples were collected from healthy women （n= 60）,benign breast disease （n= 41）, and patients with breast cancer （n=127）. Results Urinary MMP-9 and MMP-9/NGAL complex in breast disease （benign breast disease patients and breast cancer ） was higher than that in normal women （P〈0. 01）. Urinary MMP-9 and MMP-9/NGAL complex in breast cancer was higher than that in n normal women （P〈0. 01） and in patients with benign breast disease （P〈0. 05）. Conclusion Detection of urinary MMP-9, and MMP-9/NGAL complex may be a screening maker of breast disease in female population, especially for screen early breast cancer.