白色念珠菌性乳腺炎的中药治疗研究

A traditional Chinese medicine to treat mastitis caused by Candida albicans

目的以健康泌乳奶山羊为实验动物模型,观察中药对白色念珠菌性乳腺炎的治疗效果,为进一步研究奶牛白色念珠菌性乳腺炎的治疗提供试验依据。方法将30头奶山羊分为发病用药组,发病未用药组和健康对照组。给发病用药组和发病未用药组同时乳导管注射1 ml的白色念珠菌的菌液,观察炎症反应。并从患羊乳汁中分离白色念珠菌。给发病用药组用水煎的自拟中药方剂灌胃,连续用药后观察症状。分别于用药前后对30只奶山羊进行颈静脉采血用于血流变学分析,并分别于用药前后对30只奶山羊采集奶样进行乳成分分析。结果发病用药组和发病未用药组注射菌液48 h后均出现不同程度的炎症反应,并从患羊乳汁中分离到白色念珠菌。中药方剂灌胃5 d后发病用药组症状明显减轻或消失,精神状况明显好转。血流变学分析和乳成分分析结果显示,发病用药组的各项健康指标均接近或达到正常,但产奶量比健康对照组稍低。而未用药组的各项健康指标仍显著低于正常水平。结论本方剂对白色念珠菌性乳腺炎具有良好的治疗效果,为进一步研究奶牛白色念珠菌性乳腺炎的治疗提供了试验依据。

Objective Healthy lactating milk goats were used as an experimental animal model to investigate the effect of traditional Chinese medicine in treating mastitis caused by Candida albicans. Methods Thirty healthy lactating milk goats were divided into three groups： an affected group taking the medicine, an affected group not taking the medicine, and a healthy control group. One ml of C. albicans was injected into mammary ducts of the affected group taking the medicine and the affected group not taking the medicine. Inflammatory response was investigated and C. albicans was isolated from milk samples. Goats in the affected group taking the medicine were intragastrically administered an original formulation of the Chinese medicine and then the symptoms and mental state of the affected group taking the medicine were exam- ined. Blood samples were collected from the jugular vein of all 30 lactating goats both before and after medication and were then used to analyze blood theology. Milk samples from the 30 lactating goats were used to analyze milk components. Results Different levels of inflammatory response appeared 48 h after injection of C. albicans, and C. albicans was isolated from milk samples of the lactating goats that were ill. Five days after the original formulation of the Chinese medicine was intragastrically administered, the symptoms of the affected group taking the medicine were relieved or disappeared, and the mental state of the group also improved markedly. Blood rheology and milk component analysis showed that all the health indicators of the affected group taking the medicine were close to or at normal levels. However, the group had a slightly lower yield of the milk than the healthy control group. Conclusion These data suggested that this original formulation of the Chinese medicine was effective in treating mastitis caused by C. albicans. This medicine is particularly effective in treating mastitis caused by C. albicans.