OPN和B7-H4在上皮性卵巢肿瘤中的表达及意义

Expressions of osteopontin and B7-H4 in epithelial ovarian neoplasm and their significance

背景与目的:上皮性卵巢癌的发生涉及多方面的因素。近年的研究表明骨桥蛋白(osteopontin,OPN)与多种肿瘤的发生发展有关,但其在卵巢癌中的研究较少;而B7-H4是新近发现的肿瘤标志物,其在卵巢癌中的研究也很少。本研究旨在探讨卵巢上皮性肿瘤中OPN和B7-H4的表达及其临床意义。方法:采用免疫组化法检测15例正常卵巢组织、20例卵巢良性肿瘤组织、20例卵巢交界性肿瘤组织、40例卵巢癌组织标本中OPN和B7-H4的表达情况,分析两者表达与卵巢癌临床病理特征的关系。结果:OPN和B7-H4在上皮性卵巢癌组织中的表达高于卵巢交界性及良性、正常卵巢组织,各组之间的差异有统计学意义(P<0.05)。OPN和B7-H4在低分化卵巢癌中的阳性率均显著高于高、中分化卵巢癌(P<0.05),Ⅲ～Ⅳ期卵巢癌中的阳性率亦显著高于Ⅰ～Ⅱ期卵巢癌(P<0.05)。OPN阳性者伴有较高的淋巴结转移率(P<0.05),而与年龄、组织学类型无关;B7-H4在卵巢浆液性癌中的阳性率显著高于黏液性癌(P<0.05),而与年龄、淋巴结转移等无关。结论:OPN、B7-H4在上皮性卵巢癌中表达升高,可为卵巢恶性肿瘤的诊断提供参考。

Background and Objective：Epithelial ovarian cancer involved a number of factors.Recent studies have shown that osteopontin （OPN） was related to carcinogenesis and development of a variety of tumors,but few studies on ovarian cancer were reported.B7-H4 is a newly identified tumor marker in ovarian cancer.This study was to explore the expressions of OPN and B7-H4 and their clinical significance in epithelial ovarian tumors.Methods：The expressions of OPN and B7-H4 in 15 cases of normal ovarian tissue,20 benign ovarian tumor tissue,20 borderline ovarian tumor tissue,and 40 ovarian cancer tissue were detected by immunohistochemistry,and the relationship of the expressions of OPN and B7-H4 to clinical and pathologic features of ovarian cancer was analyzed.Results：The expressions of OPN and B7-H4 were higher in the ovarian cancer than in the borderline ovarian tumor and the benign tumor,and the difference was significant （P〈0.05）.The positive rates of OPN and B7-H4 were significantly higher in the poorlydifferentiated ovarian cancer than in the moderately-and well-differentiated ovarian cancer （P〈0.05）,and the expressions were significantly lower in Stages Ⅰ and Ⅱ of ovarian cancer tissues than in Stages Ⅲ and Ⅳ （P 〈 0.05）.The positive rate of OPN was statistically associated with lymph node metastasis （P 〈0.05） but not with age or histologic type.The positive rate of B7-H4 were significantly higher in the ovarian serous carcinoma than in the mucous carcinoma （P〈0.05）,but was not statistically related to age and lymph node metastasis.Conclusion：The expressions of OPN and B7-H4 increased in epithelial ovarian cancer,which could provide a reference for the diagnosis of ovarian malignant tumor.