珠海市海-陆交错带水环境原核生物多样性

Prokaryote diversity in water environment of land-ocean ecotone of Zhuhai City

采用16S rRNA基因PCR-RFLP分析技术,对珠海市海-陆交错带的海水和地下水进行原核生物多样性分析,并将所得数据与国际基因数据库Genbank进行相似性比较及聚类分析.结果表明:海水细菌以变形菌居多,其次是古菌和芽单胞菌,以及Candidate division OP3、OP8和浮霉菌等;海-陆交错带地下水细菌则以古菌居多,其次是变形菌和鞘脂杆菌,以及Candidate division OP3、放线菌和假单胞菌等.海-陆交错带地下水中大部分优势类群与源于海洋的不可培养细菌具有很高的相似性.地下水样中存在大量能降解有机质和具有净化水体潜力的细菌,说明在长期演化下,海-陆交错带兼备了海洋生态系统和陆地生态系统的特征.

By constructing 16S rDNA clone library with PCR-RFLP,the prokaryote diversity in the seawater and groundwater of land-ocean ecotone of Zhuhai City was investigated,and the similarity and cluster analyses were implemented with the database of the sequences in Genbank.In the seawater,Proteobacteria was dominant,followed by Archaeon,Gemmatimonadetes,Candidate division OP3 and OP8,and Planctomycetes,etc.;while in the groundwater,Archaeon was dominant,followed by Proteobacteria,Sphingobacteria,Candidate division OP3,Actinobacterium,and Pseudomonas.The dominant taxa in the groundwater had high similarity to the unculturable groups of marine microorganisms.Large amount of bacteria capable of degrading organic matter and purifying water body existed in the groundwater,suggesting that after long-term evolution,the land-ocean ecotone of Zhuhai City had the characteristics of both land and ocean.