骨形态发生蛋白-7对马兜铃酸诱导人肾小管上皮细胞转分化的影响

Effect of Bone Morphogenetic Protein-7 on Aristolochic Acid Induced Renal Tubular Epithelial Cells Trans-differentiation

目的探讨骨形态发生蛋白-7(bone morphogenetic protein-7,BMP-7)对马兜铃酸(Aristolchic acid,AA)诱导的肾小管上皮细胞转分化的拮抗作用,以期为马兜铃酸肾病(Aristolchic acid nephropathy,AAN)寻找治疗新措施。方法应用不同浓度BMP-7(75ng/mL、150ng/mL、300ng/mL)处理经AA(10μg/mL)诱导转分化的体外培养人近端肾小管上皮细胞株(HK-2细胞),然后应用逆转录-聚合酶链反应(RT-PCR)及Western blotting技术分别检测α-SMA mRNA及蛋白的表达水平。结果BMP-7呈剂量依赖方式逆转AA诱导的HK-2细胞α-SMA基因及蛋白的表达。结论BMP-7可以通过抑制马兜铃酸诱导的人肾小管上皮细胞转分化从而可能成为AAN的防治潜在新药。

Objective To observe the effect of bone morphogenetic protein-7 （BMP-7） on aristolchic acid induced renal tubular epithelial cell trans-differentiation to look for new therapeutic approach for aristolchic acid nephropathy （AAN）. Methods In vitro cultured human proximal renal tubular epithelial cell line HK-2 cells were treated with different concentrations of BMP-7 （75 ng/mL, 150 ng/mL and 300 ng/mL） after trans-differentiation of the cells was induced by AA （10 μg/mL）. Levels of α-SMA mRNA and protein expressions were detected by semi-quantitative reverse transcription polymerase chain reaction （RT-PCR） and Western blotting respectively. Results BMP-7 reversed the AA inducing α-SMA expressions in HK-2 cells in a dose-dependent manner. Conclusion BMP-7 can inhibit the trans-differentiation of human renal tubular epithelial cell induced by AA, thereby might be a new potential drug for AAN prevention and treatment.