脑络欣通和左归丸药物血清对体外培养的大鼠神经干细胞增殖分化的作用

Effects of Drug-contained Sera of Naoluo Xintong and Zuogui Pill on the Proliferation and Differentiation of in vitro Cultured Neural Stem Cells in Rats

目的探讨脑络欣通药物血清和左归丸药物血清对体外培养的大鼠胚胎神经干细胞(neural stem cell,NSC)增殖分化的作用。方法分别采用含10%脑络欣通药物血清和含10%左归丸药物血清的培养基培养大鼠胚胎NSC,观察其促增殖效应和诱导分化效应,应用相差显微镜和免疫荧光染色对其进行比较观察。结果脑络欣通药物血清和左归丸药物血清均可诱导绝大多数NSC分化成神经元、星形胶质细胞和少突胶质细胞。此外,两者还能在一定程度上促进培养的NSC生长。前者诱导NSC分化的进程虽比后者要慢,但诱导NSC向神经元方向分化的比率较高,其分化的神经元在细胞形态学上与发育成熟的神经元更为接近;后者促进NSC生长的效应较为显著。结论脑络欣通和左归丸均能促进体外培养的NSC生长和分化,两者作用有一定差异;益气活血治法和补肾生髓治法对NSC进行诱导分化具有一定的可行性。

Objective To investigate the effects of drug-contained sera （DCS） of Naoluo Xintong （NLXT） and Zuogui Pill （ZGP） on the proliferation and differentiation of in vitro cultured embryonic neural stem cells （NSCs） in rats. Methods Rat＇s embryonic NSCs were cultured in medium supplemented with 10% DCS with NLXT and ZGP separately, the effects of DCS in enhancing proliferation and inducing differentiation were observed and compared by phase contrast microscopy and immunofluorescence staining. Results Majority of NSCs were induced to neurons, astrocytes or oligodendrocytes in medium supplemented with either DCS-NLXT or DCS-ZGP, with the growth of them promoted to some extent, However, DCS-NLXT induced the differentiation rather slowly but with the differentiated neurons more resemble to the mature neuron in morphology, while DCS- ZGP promoted the stem cell growth more effectively. Conclusion Both NLXT and ZGP could promote the differentiation and growth of in vitro cultured NSCs, but with exiguous difference. It is feasible to induce the proliferation and differentiation of NSCs by way of using Chinese medicine drug-therapy for supplementing qi and activating blood circulation as well as that for tonifying Shen to generate marrow.