没食子酸丙酯Fe(Ⅲ)、Cr(Ⅲ)、Cu(Ⅱ)配合物的合成、表征及模拟超氧化物歧化酶

Synthesis,Characterization and Simulation of SOD of M(M:Fe(Ⅲ),Cr(Ⅲ),Cu(Ⅱ))-Propyl Gallate Complexes

研究了没食子酸丙酯在水溶液中分别与Fe3+、Cr3+、Cu2+生成没食子酸丙酯-铁(PG-Fe)、没食子酸丙酯-铬(PG-Cr)、没食子酸丙酯-铜(PG-Cu)这3种金属配合物(PG-M)的反应。通过紫外光谱、核磁共振、红外光谱、质谱、元素分析、摩尔电导率和循环伏安法等测试技术对3种金属配合物进行了表征。确定3种金属配合物的分子式分别为[C10H10O5]2Fe.H2O、[C10H10O5]3Cr.2H2O、[C10H10O5]2Cu.2H2O。红外光谱分析表明,中心金属原子与酚羟基发生配位,形成Ar—O—M键。电化学数据显示,PG-M的抗氧化性优于PG配体,且PG-Cu>PG-Cr>PG-Fe。用改进的核黄素光还原NBT法检测了3种金属配合物的模拟超氧化物歧化酶(SOD)活性。结果表明,3种金属配合物均有一定的催化歧化超氧阴离子自由基(O2-.)活性,均可被称作超氧化物歧化酶活性中心的模拟配合物。

The interactions of Fe^3 ＋, Cr3^＋, Cu^2 =with propyl gallate （PG） in water were studied. The solid complexes of PG-Fe, PG-Cr, PG-Cu were characterized by UV-Vis, ^1H NMR, IR, MS, elemental analysis, molar conductance and cyclic voltammetry. The results support the formation of the complexes with the formulae [ C10H10O5 ] 2Fe· H2 O, [ C10 H10O5 ] 3 Cr· 2H2O and [ C10 H10O5 ] 2 Cu· 2H2O for investigated metal cations. The IR spectra show that the phenol hydroxyl group was coordinated to the metal center to form the Ph-O-M bond. The electrochemical data suggest that the metal complexes were more effective antioxidants than PG ligand, and the antioxidative property follows the order： PG-Cu 〉 PG-Cr 〉 PG-Fe. The superoxide dismutase（SOD） like activity of the metal complexes was measured by means of a reduction of NBT by VB2. The results indicate that the three metal complexes could catalyze the disproportionation of the superoxide anion free radical （ O2^- · ）, and could be used as the active center model complexes of SOD.