涿鹿柳蒿中氨基酸含量的测定

Determination of Amino Acid in Artemisia selengensis Turcz. by HPLC

目的：建立柳蒿中氨基酸含量测定的高效液相色谱方法，并测定柳蒿中氨基酸的含量。方法：Hy—persil AAA-ODS色谱柱（2．1mm×150mm5μm），采用梯度洗脱，检测波长330nm，柱温40℃。结果：线性范围：12．5-50pmoL·μL，平均回收率：96．2％～104．6％。结论：该方法快捷、稳定、重复性好，可用于柳蒿中氨基酸的含量测定。

Objective：To investigate HPLC method in measuring Amino Acid in Arternisia selengensis Turcz. ,and determine amino acid in Arternisia selengensis Turcz.. Methods： Eclipse AAA column（4. 6mm ×150mm, 5μm）, using gradient solution and with detection wavelength at 330 nm, the temperature of column was 40℃. Results：The method was liner within the range of 12.5-50pmoL · μL^-1 ,The average recovery of assay was 96.2%-104.6%. Conclusion：This method is fast,reliable and accurate. It can be used for determining Amino Acid in Arternisia selengensis Turcz..