吴茱萸提取物的抗氧化作用

The Anti-oxidation of the Extract from Evodia Rutaecarpa(Juss.)Benth.

目的:研究吴茱萸提取物清除羟自由基(.OH)和超氧阴离子自由基(.O2-)的作用。方法:用硫酸亚铁和过氧化氢反应产生.OH;用邻苯三酚自氧化法产生.O2-。用分光光度法测定吴茱萸正丁醇提取物1、正丁醇提取物2和乙醚提取物对.OH和.O2-的清除作用。结果:三种提取物及Vc清除.OH的IC50分别为145.0、141.0、335.0、2.3μg.mL-1;正丁醇提取物1清除.O2-的作用无法测定,吴茱萸正丁醇提取物2、乙醚提取物及Vc清除.O2-的IC50分别为270.00、.185、0.057 5μg.mL-1。结论:本研究中的三种吴茱萸提取物均有一定的抗氧化作用并呈量效关系。吴茱萸脂溶性成分清除.O2-的作用非常显著,与Vc有相似作用。

Objective.. To study the removal function of exfraet from Evodia rutaecarpa （Juss.）on hydroxyl radical（ ·OH ）and Superoxide anion radical（ ·O2 ）. Methods： Kreaetion of ferrous sulfate and hydrogen peroxide was used to generate ·OH, with the pyrogallol autoxidation method producing ·O2^- The removing ability of n-butanol extract-1 ,n-butanol extract-2 and ether extract was determined by spectrophotometry. Re-suits.. IC50 of scavenging · OH for these three kinds of extracts and VC were 145.0,141.0,335.0,2.3μg ·mL^-1 respectively;but the ability of removing · O2- of n-butanol extract-1 could not be determined,lCs0 of the rest three were 270.0,0. 185 and 0. 057 5μg · mL^-1 respectively. Conclusion：Three extracts from Evodia rutaecarpa （Juss.）Benth. have some anti-oxidation,and showed dose-effect relationship. The ability of removing · O2 for liposolubles was very remarkable,and it had similar ability with that of Vc.