小鼠实验性缺氧脑和肺组织MDA、LD及LDH的变化(英文)

Changes of MDA,LD and LDH in Brain and Lung with Experimental Anoxia in Mice

目的:探讨小鼠实验性缺氧脑和肺组织丙二醛(Malondialdehyde,MDA)、乳酸(Lactic acid,LD)含量和乳酸脱氢酶(Lactate dehydrogenase,LDH)活性变化。方法:18只小鼠随机分为3组,①正常对照组;②急性缺氧组;③急性一氧化碳染毒组。分别测定脑和肺组织MDA、LD、LDH含量和活性。结果:急性缺氧组和急性一氧化碳染毒组小鼠脑和肺组织MDA和LD含量显著增高。LDH活性在两实验组小鼠肺组织显著降低,而在脑组织无显著性改变。结论:MDA和LD含量在缺氧早期脑和肺组织是易变化性指标,其改变可能与该组织损伤程度有关。肺较脑对缺氧产生的自由基损伤更敏感。

Objective： To determine the levels of malondialdehyde（MDA）, Lactic acid, （LD） and activity of Lactate dehydrogenase（LDH） for mice with experimental anoxia. Methods： 18 mice were randomly divided into three groups. Mice in group 1 served as no hypoxic control,mice in Group 2 were subjected to acute hypo- baric hypoxic exposure and mice in group 3 were subjected to CO poisoning. All animals were killed after 40 min. Brain and lung specimens were obtained to determine the tissue level of MDA, LD and activity of LDH. Results：MDA and LD levels were significantly increased in mice of group 2 and group 3 compared with that of control group both in brain and lung. The activity of LDH was significantly lower in lung for mice of both groups 2 and 3 compared with that of control group, but there was no significant change in brain for the mice of rest two groups. Conelusion：MDA and LD are earlier changing indexes with anoxia in brain and lung,and are related to the damage degree of tissue. Lung is more sensitive for exposure to free radical-generation system than brain.