女性患者淋球菌对抗生素的多重耐药性分析

Analysis on the antibiotic multi-drug resistance of neisseria gonorrhoeae isolated from female patients

目的了解本地区女性淋球菌感染患者对抗生素的多重耐药状况,为临床合理用药提供实验依据。方法应用K-B琼脂扩散法分析女性感染淋球菌对不同类别抗生素的敏感性,筛选出多重耐药菌株;用琼脂稀释法测定青霉素、四环素、环丙沙星对淋球菌的最小抑菌浓度(MIC);碘量法检测产青霉素酶淋球菌(PPNG)。结果112例女性患者标本经分离、培养、鉴定的淋球菌为86株。从86株淋球菌中筛选出58株(67.44%)多重耐药株。青霉素耐药率为51.16%(44/86),四环素耐药率61.25%(49/80),其中质粒介导的PPNG和高度耐四环素(TRNG)菌株的阳性率分别为18.60%(16/86)和12.50%(10/80);环丙沙星耐药率92.94%(79/85)。结论本地区女性感染淋球菌出现的多重耐药状况较为严重,有必要持续监测淋球菌的耐药性。

Objective To investigate the antibiotic multi-drug resistance of neisseria gonorrhoeae （N. gonorrhoeae ） strains isolated from the local female patients and provide empirical evidences for the rational drug administration in clinic. Methods Antimicrobial susceptibility of N. gonorrhoeae to various antibiotics was analysed by K-B disk diffusion method, and strains of multi-drug resistance were screened out. The agar-dilution method was used to determine the minimum inhibitory concentration （MIC） of three antibiotics including Penicillin-G,Tetracyctine,Ciprofloxacin and that of PPNG by the tube iodine quantitative method. Results A total of 86 N. gonorrhoeae strains were isolated from 112 female patients,and 58 multi-drug resistance strains （67.44%） were obtained by the K-B method. The resistant rate for penicillin was 51.16% （44/86） with PPNG being 18.60% （16/86）. Tetracycline-resistant isolates accounted for 61.25 （49/86） with 12.50% （10/80） high level tetracycline-resistant （TRNG）,and 92.94% （79/85） of these isolates were resistant to ciprofloxacin. Conclusion The study shows that the mult-resistance of N. gonorrhoeae in female patients was severe. It is necessary to survey the antibiotic resistance of N. gonorrhoeae continiously.