微囊藻毒素检测方法改进和圆明园实际水样的测定

IMPROVEMENT OF MICROCYSTIN'S DETECTION AND DETERMINATION OF YUANMINGYUAN WATER

在比较影响藻毒素MC的检测条件后,建立了固相萃取-高效液相色谱分析水中微量藻毒素的方法.此方法对于MC-RR的准确度、精密度可达1.06%、1.35%,MC-LR的准确度、精密度为2.75%、0.42%,且在0.1～5.0μg/mL范围内线性良好,相关系数都可达到0.999.对圆明园水样进行藻毒素含量测定的结果表明,此方法可有效地检测水样中的微量微囊藻毒素.圆明园水样MC分析显示其水中含有MC-LR及MC-RR,加标回收率分别为90.4%、91.7%.

The methods for analyzing Microcystins in water using SPE and HPLC were developed by comparing the conditions which had influence on the analysis. The accuracy and precision of MC-RR were up to 1.06% and 1.35% , while MC-LR were 2.75% ,0.42% respectively, with a well linear relation at the range of 0. 1 -5.0 μg/mL and the respective correlation coefficient were all above 0. 999. In addition, the results of determining of the MC content in water form Yuanmingyuan Park showed that this method could determine the microcystins in water efficiently. The analysis of water from Yuanmingyuan Park indicated that there are MC-LR and MC-RR, the recovery of MC-LR was 90.4% , MC-RR was 91.7%.