不同证候H22荷瘤小鼠胸腺一致上下调高表达的基因

Coincident Up-regulated or Down-regulated High Expressed Genes in Thymus of H22 Tumor-Bearing Mice of Different Syndromes

目的:观察常见证候荷瘤小鼠胸腺中一致上调和下调的高表达基因。方法:采用荷瘤小鼠及其标准化四诊及辨证方法,以及GeneChip Mouse Exon1.0ST Array等技术,检测H22荷瘤小鼠在早期邪毒壅盛证、气虚证,中期阳虚证,中晚期阴虚证等4个证候胸腺中一致上调或下调者,重点关注表达量高的基因。结果:肿瘤发生后,胸腺中有34个基因表达发生显著改变。其中一致上调者20个,一致下调9个。结论:(1)Tpp2、per2基因在肿瘤发生的早期表达几乎关闭,与肿瘤发生的早期关系密切。(2)20个高表达基因,如Hey2、MMP-19等,可能在中晚期胸腺退化阶段起到特殊的作用。(3)推测这些一致上调和下调的基因与肿瘤发生及其不同阶段的关系更为密切,而非证候。

Objective： To investigate the characteristics of coincident up- or down-regulated high expressed genes in thymus of H22 tumor-bearing mice of different syndromes.Methods： The thymus gene expression in H22 tumor-bearing mice of pathogenic toxin-exuberance syndrome and qi deficiency syndrome on early stage, yang-qi deficiency syndrome on intermediate stage, qi-yin-yang deficiency syndrome on advanced stage were detected with quantitative four diagnosis and syndrome differentiation methods and GeneChip Mouse Exon 1.0 ST Array to screen out the up- and down-regulated genes in uniform, and payed close attention to the highly expressed genes. Results： There are thirty-four gone expression changed obviously after the tumor happened, twenty up-regulated and nine down-regulated. Conclusion： （1）Gone Expression of Tpp2, per2 almost closed on early stage after the tumor happened, they have a close relationship with the early stage of the tumor.（2）The twenty up-regulated genes, such as Hey2, MMP-19, may play a special role during the thymus involution on advanced stage.（3）We think these upregulated or down-regulated genes may be related to tumorigenesis and its different stage, but not the symptoms and signs.