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PPARγ在肝外胆管癌中的表达及其意义 被引量：1

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摘要 目的观察PPARγ mRNA和蛋白在肝外胆管癌组织中的表达,并探讨其与肝外胆管癌临床病理特征之间的关系。方法采用RTPCR和Western印迹检测PPARγ mRNA和蛋白在30例肝外胆管癌组织中的表达情况。结果RT-PCR法检测结果显示肝外胆管癌组织中,淋巴结转移组和无淋巴结转移组PPARγ mRNA表达分别为0.47±0.06和0.24±0.07,差异有统计学意义(P<0.01);低分化组和高中分化组PPARγ mR-NA表达分别为0.3±0.19和0.17±0.01,差异无统计学意义(P>0.05);Ⅱ、Ⅲ期和Ⅰ期肝外胆管癌组织PPARγ mRNA表达分别为0.63±0.35和0.63±0.28,差异无统计学意义(P>0.05)。Western印迹结果显示肝外胆管癌组织中,淋巴结转移组和无淋巴结转移组PPARγ蛋白表达水平分别为2.0±0.44和0.93±0.21,差异有统计学意义(P<0.01);低分化组和高中分化组中,PPARγ蛋白表达分别为0.87±0.23和0.96±0.15,差异无统计学意义(P>0.05)。Ⅱ、Ⅲ期和Ⅰ期,PPARγ蛋白表达分别为2.45±0.54和1.95±0.24,两组差异无统计学意义(P>0.05)。结论PPARγ可能对肝外胆管癌的发生和发展起促进作用。
作者 刘兵 王文斌 王安峰 吕海涛 刘明
机构地区 河北医科大学第三医院放疗科 河北医科大学第二附属医院
出处 《中国老年学杂志》 CAS CSCD 北大核心 2010年第4期450-452,共3页 Chinese Journal of Gerontology
基金 河北省卫生厅医学科学研究重点课题计划指令性项目(08141)
关键词 PPARΓ 肝外胆管癌 逆转录-聚合酶链式反应 免疫印迹
分类号 R735.8 [医药卫生—肿瘤]
  • 相关文献

参考文献10

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二级参考文献11

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共引文献9

同被引文献19

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中国老年学杂志
2010年 第4期

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