人前列腺癌PC3细胞亚群的分离及其生物学行为分析

Isolation and biological behaviour analysis of prostatic carcinoma PC3 cell subpopulation

目的研究人前列腺癌PC3肿瘤细胞系中是否存在具有干细胞特征的细胞亚群,并对其形态及克隆生长特征进行分析,同时探讨染料Rho123着色强度是否可用于区分不同的肿瘤细胞亚群。方法采用Rho123染色后流式细胞仪分析;Rho123与DAPI复染后荧光显微镜下形态学观察及细胞克隆生长特征分析等方法。结果PC3细胞中存在对Rho123着色显著差异的两类细胞亚群,其形态结构、增殖能力及克隆生长方式均有明显差别。结论人前列腺癌PC3细胞系中存在具有干细胞特性的细胞亚群,对Rho123拒染的特性可用于区分肿瘤干细胞与其他肿瘤细胞。

Objective To identify the characteristic of cancer stem-like cells in prostatic carcinoma PC3 cell line,and analyze the morphous and clone growth,investigate the method of discriminating the different tumor cell subpopulation by Rhodamin123 dye. Methods The PC3 cells stained with Rhodamin123 dye were analyzed by flow cytometry;the cells morphous and clone growth stained with Rhodamin123 and DAPI were observed by fluorescent microscope. Results Two different tumor cell subpopulation existed in PC3 cell line,and the morphous,reproductive activity and colon growth were significantly different in the two cell subpopulation. Conclusions Cancer stem-like cell subpopulation exists in prostatic carcinoma PC3 cell line,the character of some cells excluding Rhodamin123 dye can be used to discriminate tumor stem cells and other tumor cells.