P13K／Akt信号通路在吡咯喹啉醌促雪旺细胞增殖中的作用

The roles of PI3K/Akt pathway in proliferation of Schwann cells promoted by Pyrroloquinoline Quinine

目的探讨磷脂酰肌醇-3激酶／蛋白激酶B（phosphoinositide-3 kinase／Akt，P13K／Akt）信号通路在吡咯喹啉醌促雪旺细胞增殖过程中的作用。方法体外分离培养雪旺细胞，S-100免疫荧光鉴定；Westernblot检测P13K下游因子Akt磷酸化激活形式（p-Akt）的表达，并通过P13K激酶抑制剂（wortmannin）阻断该通路后p-Akt的表达情况。结果吡咯喹啉醌可使雪旺细胞发生形态学变化，加入吡咯喹啉醌后30min即可检测到p-Akt的表达，4h达高峰，12h基本无表达；吡咯喹啉醌在1—100nmol／L范围内可使p-Akt表达增加；加入wortmannin阻断P13K后p-Akt上调表达消失（P〈0．05）。结论吡咯喹啉醌可使雪旺细胞发生形态学变化，P13K／Akt信号通路在吡咯喹啉醌促雪旺细胞增殖过程中发挥重要作用。

Objective To investigate the roles of PI3K/Akt signal pathway in Schwann cells proliferation promoted by pyrroloquinoline quinine （PQQ）. Methods Schwann cells were cultured and purified in vitro. The purity was identified by immunofluorescence of S-100; the expression of Akt and phosphorylated-Akt（p-Akt）were detected by western blot, and the expression of p-Akt after blocking the PI3K signal transduetion pathway by PI3K inhibitor wortmannin was detected by western blot. Results Morphological change was observed in PQQ-treated Schwann cells, p-Akt was detected 30rain after PQQ treated, reached the peak at 4h, and disappeared 12 h later. 1-100 nmol/L PQQ could up-regulate the expression of p-Akt; this up-regulated expression of p-Akt was inhibited by wortmannin （ P 〈 0.05）. Conclusions PQQ could affect morphology of Schwann cells and activation of Akt. It indicates that PI3K/ Akt signal pathway might be involved in Schwann cells proliferation promoted by PQQ.