摘要
目的研究重组小鼠IL-13对肺成纤维细胞的作用及其机制。方法取生长良好的NIH/3T3,给予无血清培养基饥饿24 h,在培养液中加入IL-13。MTT法、RT-PCR等方法测定肺成纤维细胞的增殖及TGF-β1、PDGF-AA、-αSMA等因子的表达。MTT法观察TGF-β单克隆抗体干预后,IL-13对NIH/3T3细胞增殖反应的影响。结果与对照组相比,IL-13促进NIH/3T3细胞增殖(P<0.05)。IL-13能够显著地促进NIH/3T3细胞中TGF-β1和PDGF-AA mRNA的表达,刺激NIH/3T3分泌-αSMA。TGF-β单克隆抗体能抑制IL-13诱导引起的NIH/3T3细胞增值。结论IL-13能促进成纤维细胞的增殖,其可能的机制是通过促进成纤维细胞分泌TGF-β1和PDGF-AA来发挥作用。
Objective To study the effect and mechanism of recombinant interleukin（IL）-13 on lung fibroblasts in mice. Methods The method of MTT was adopted to detect the influence of recombinant IL-13 on proliferation of NIH/3T3 cells. The expressions of transforming growth factor- β1 （TGF-β1）, platelet-derived growth factor-AA（PDGF-AA） and α-smooth muscle acting protein （α-SMA） genes of NIH/3T3 cells treated by recombinant murine IL-13 were detected by RT-PCR and MTT. The untreated sample served as the control. The influence of IL-13 on NIH/3T3 cells after the intervention of monoclonal anti-TGF-β antibody was observed as well. Results The recombinant IL-13 promoted the proliferation of NIH/3T3 cells. IL-13 significantly enhanced the expressions of TGF-β1 and PDGF-AA genes. There was only a slight upreguluation of α-SMA induced by IL-13. Fibroblast proliferation induced by IL-13 could be significantly restrained by anti-TGFβ antibody in vitro （P〈 0. 01）. Conclusion Recombinant IL-13 of mice can promote the proliferation of tung fibroblasts through inducing the higher expressions of TGF-β1 and PDGF-AA.
出处
《江苏医药》
CAS
CSCD
北大核心
2010年第3期316-318,共3页
Jiangsu Medical Journal
