雾化吸入氟化碳对急性肺损伤大鼠肺表面活性物质及其相关蛋白-A和TNF-α的影响

Effects of perfluorocarbon aerosol inhalation on the lung surfactant and surfactant-related protein A and TNF-α in rats with acute lung injury

目的探讨雾化吸入氟碳化合物(PFC)对急性肺损伤大鼠肺表面活性物质、肺表面活性物质相关蛋白-A(SP-A)与肿瘤坏死因子α(TNF-α)的影响。方法A组采用腹腔注射脂多糖(LPS)诱导大鼠急性肺损伤模型,并雾化吸入PFC进行干预;B组以生理盐水代替LPS作为对照。观察PFC对鼠动脉血气、支气管肺泡灌洗液(BALF)中总蛋白、双饱和磷脂酰胆碱(DPPC)、SP-A、TNF-α及动物死亡率的影响。结果与B组相比,A组PFC吸入明显提高动脉血氧分压(PaO2),增加BALF中SP-A及DPPC的浓度,降低BALF中总蛋白与TNF-α浓度,降低24 h内的死亡率(P<0.01)。结论PFC雾化吸入可通过增加SP-A及DPPC浓度、降低BALF中总蛋白与TNF-α浓度降低急性肺损伤大鼠死亡率。

Objective To investigate the effects of perfluorocarbon （PFC） aerosol inhalation on lung surfactant,surfactant-related protein A （SP-A） and tumor necrosis factor（TNF）-α in rats with acute lung injury （ALI）. Methods ALI in group A was induced by intraperitoneal injection of lipopolysaccharide（LPS） and intervened with PFC. SP-A, TNF-α and lung surfactant concentration in bronchoalveolar lavage fluid （BALF） were measured. The results were compared with those in group B, in which normal saline was used instead of LPS as the control. Results Compared with group B, PFC aerosol inhalation in group A significantly reduced death incidence with the increases in SP-A and lung surfactant levels, but a decrease in TNF-α concentration in the BALF （P〈0. 01）. Conclusion PFC inhalation increases the survival rate of ALI rats by increasing SP-A and lung surfactant and decreasing TNF-α of BALK