吡格列酮对兔动脉粥样硬化血管壁ICAM-1表达的影响

Effect of pioglitazone on expression of ICAM-1 in an atherosclerotic rabbit aorta

目的:探讨吡格列酮对动脉粥样硬化(Atherosclerosis,AS)血管壁细胞间粘附分子(Intercellular adhesion molecule-1,ICAM-1)表达的影响。方法:新西兰兔随机分成5组:A:正常对照,B:正常+低剂量吡格列酮(Pioglitazone,PIO),C:病理对照,D:病理+低剂量PIO,E:病理+高剂量PIO。采用免疫组化及逆转录聚合酶链反应(RT-PCR)的方法观察吡格列酮血管壁ICAM-1蛋白及mRNA表达的影响。结果:AS斑块内有ICAM-1蛋白和mRNA高表达,PIO可抑制ICAM-1蛋白和mRNA表达(P<0.05),高剂量较低剂量抑制程度更大(P<0.05)。结论:PIO可抑制AS斑块ICAM-1的mRNA和蛋白表达,进而发挥抗AS炎症的作用。

Objective：To explore the effect of pioglitazone （PIO） on expression of intercellular adhesion molecule-1 （ICAM-1） in atherosclerotic rabbit aorta. Methods ： Newzealand white rabbits were divide into 5 groups ： normal control, normal＋low dose of PIO, and AS control,AS＋low dose of PIO,AS＋high dose of PiO. Immunohistochemistry was used to detect the protein of ICAM-1. Reverse transcription -polymerase chain reaction （RT-PCR） was used to detect the mRNA of ICAM-1. Results：ICAM-1 protein and mRNA in AS plaques. PIO could inhibit the expression of ICAM- 1 protein and mRNA （p〈0.05）. The effect of high dose was more than that of were highly expressed low dose（P〈0.05 ）. Conclusion： PIO could inhibit the expression of ICAM-1 and further produce its anti-inflammatory effect on AS.