白芍总苷对慢性非细菌性前列腺炎大鼠IFN-γ、TNF-α和IL-10表达影响的实验研究

Effects of total glucosides of paeony on the expression of IFN-γ,TNF-αand IL-10 in the rat model for chronic abacterial prostatitis

目的:观察白芍总苷(Total glucosides of paeony,TGP)对慢性非细菌性前列腺炎(Chronic abacterial prostatitis,CAP)大鼠干扰素-γ(Interferon-γ,IFN-γ),肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-γ)和白细胞介素-10(Interleukin-10,IL-10)表达的影响,并初步探讨其作用机制。方法:将40只老龄SD大鼠随机分为正常对照组、CAP模型组、CAP+TGP低剂量治疗组、CAP+TGP高剂量治疗组,每组10只。观察大鼠前列腺病理形态学改变,免疫组化法检测IFN-γ、TNF-α在各组大鼠前列腺中的表达,ELISA法定量分析各组大鼠前列腺中IL-10的表达。结果:(1)CAP大鼠模型的组织病理学改变符合CAP的特点,治疗组炎症明显消退;(2)CAP模型组IFN-γ、TNF-α和IL-10表达强度显著高于正常对照组;(3)TNF-α、INF-γ和IL-10蛋白表达随TGP呈剂量相关性降低(P<0.01);(4)各组大鼠之间INF-γ、TNF-α和IL-10的比值有统计学意义(P<0.01)。结论:TGP对CAP大鼠具有治疗作用,其机制可能与其调节CAP大鼠异常的免疫功能,平衡细胞因子的产生有关。

Objective： To investigate the influence of TGP on IFN- γ ,TNF-α and IL-10 in prostatic tissue of CAP rats. Methods： 40 aged sprague dawley rats were randomly divided into four groups： the control group （10 rats ） , the rat model for CAP group （10 rats）, the rat model for CAP treated with low dose TGP group （ 10 rats） and the rat model for CAP treated with high dose TGP group （ 10 rats）. The changes of prostate gland inflammation was investigated by HE stain. The expression of IFN- γ and TNF-α in rat prostatic tissue was detected by immunohistochemistry （sp）. The expression of IL-10 in rat prostatic tissue was detected by enzyme linked immunosorbent assay（ELISA ）. Results ： （ 1 ） The changes in histopathology in the animal model were similar to those in clinical CAP and the inflammation of treatment group obvious change for the better; （2） The IFN- γ , TNF-α and IL-10 in the rat model for CAP group were higher than the control group ; （ 3 ） The IFN- γ , TNF-α and IL-10 expression reduces along with the TGP dosage increases in the rat model for CAP （P〈0.01）; （4） Between each group the IFN/IL-10 and TNF/IL-10 have statistics significance （P〈0.01）. Conclusion：TGP has therapeutic effects on CAP rats. The mechanisms may be associated with its ability to ameliorate the abnormal immunological functions and cytokine secretion in CAP rats.