葡萄糖对INS-1细胞活性氧及PTEN表达的调控

Regulation of Glucose upon Reactive Oxygen Species and PTEN Expression in INS-1 Cells

目的:研究葡萄糖刺激胰岛β细胞后,是否通过活性氧(ROS)调控第10号染色体缺失的磷酸酶与张力蛋白同源物基因(PTEN)表达而参与胰岛素分泌信号通路的调控。方法:用不同浓度葡萄糖和H2O2短时间干预胰岛细胞系(INS-1细胞),分别检测细胞分泌胰岛素、细胞内ROS浓度及PTENmRNA表达。结果:(1)随着刺激的葡萄糖、H2O2浓度升高,INS-1细胞内ROS浓度和分泌的胰岛素逐渐升高。(2)随着葡萄糖刺激加大或H2O2浓度逐渐升高,INS-1细胞内PTENmRNA表达先受到抑制,但当刺激浓度超过一定范围后,抑制作用消失。结论:葡萄糖可能通过刺激胰岛细胞内ROS生成,以低浓度ROS作为信号分子抑制PTEN表达,成为调控胰岛素分泌的机制之一。

Objective：To study effect of reactive oxygen species （ROS） ,induced from hyperglucose,on the expression of PTEN gene in INS-1 cell line. Methods：The cultured INS-1 cell line was exposed to different concentrations of glucose and H2O2 for a short time. Then,concentrations of secreted insulin,ROS and phosphatase and tensin homologue detetell on chromososme ten （PTEN） mRNA levels in cultured cells were detected. Results： Concentrations of ROS in INS-1 cells and secreted insulin in- creased along with increased glucose or H2O2 exposure. The PTEN mRNA expression decreased along with increased glucose or H2O2 exposure,but when glucose or H2 O2 were above a certain concentration, their inhibition on PTEN mRNA expression disappeared. Conclusion： Low ROS derived from glucose metabolism can inhibit the PTEN gene expression,which may be one of insulin secretion mechanisms in INS-1 cells induced by glucose stimulation.